Tracking operator state fluctuations in gene expression in single cells

Banerjee, B. ; Balasubramanian, S. ; Ananthakrishna, G. ; Ramakrishnan, T. V. ; Shivashankar, G. V. (2004) Tracking operator state fluctuations in gene expression in single cells Biophysical Journal, 86 (5). pp. 3052-3059. ISSN 0006-3495

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00063...

Related URL: http://dx.doi.org/10.1016/S0006-3495(04)74354-4

Abstract

We report the results of operator state fluctuations in gene expression for the entire bacterial growth cycle, using single-cell analysis and synthetic unregulated and negative-feedback transcription regulatory gene circuits. In the unregulated circuit, during the cell cycle, we observe a crossover from log-normal-to-normal distribution of expressed proteins and an unusual linear dependence of their standard deviation on the mean gene expression levels. With negative-feedback circuits we find the existence of bimodality as the cell cycle progresses. We suggest that such long-tail and bimodal distributions may be used as selection mechanisms in developmental switches and for assigning cell identity.

Item Type:Article
Source:Copyright of this article belongs to Biophysical Society.
ID Code:40859
Deposited On:25 May 2011 11:19
Last Modified:17 May 2016 22:47

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