A study on cryopreservation of cultured rabbit periodontal ligament cells

Abstract

Cultured rabbit periodontal ligament cells were subjected to short term cryopreservation in liquid nitrogen, for a period of 84 hours and 168 hours, to study the effect of cryopreservation on coll viability and culturing ability. The vital cell count performed by Trypan Blue exclusion was 14.93 x 10(0) cells/ml in vial A and 9.11 x 10(6) cell/ml in vial B, before cryopreservation. The loss of viability was minimal--vital cell count being 14.64 x 10(6) cells/ml in vial A and 8.87 x 10(6) cells/ml in vial B after 84 hrs of cryopreservation and 14.6 x 10(6) cells/ml in vial A and 8.82 x 10(6) cells/ml in vial B after 168 hours of cryopreservation. The cryopreserved cells after thawing could grow again in cultured.