Kusurkar, Tejas S. ; Tandon, Ishita ; Sethy, Niroj Kumar ; Bhargava, Kalpana ; Sarkar, Sabyasachi ; Singh, Sushil Kumar ; Das, Mainak (2013) Fluorescent silk cocoon creating fluorescent diatom using a “water glass-fluorophore ferry” Scientific Reports, 3 . Article ID 3290, 9 pages. ISSN 2045-2322
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Official URL: http://www.nature.com/srep/2013/131121/srep03290/f...
Related URL: http://dx.doi.org/10.1038/srep03290
Abstract
Fluorophores are ubiquitous in nature. Naturally occurring fluorophores are exceptionally stable and have high quantum yield. Several natural systems have acquired fluorescent signature due to the presence of these fluorophores. Systematic attempt to harvest these fluorophores from natural systems could reap rich commercial benefit to bio-imaging industry. Silk cocoon biomaterial is one such example of natural system, which has acquired a fluorescent signature. The objective of this study is to develop simple, rapid, commercially viable technique to isolate silk cocoon membrane fluorophores and exploring the possibility of using them as fluorescent dye in bio-imaging. Here, we report an innovative water glass (Na2SiO3) based strategy to isolate the silk cocoon fluorophores. Isolated fluorophore is majorly quercetin derivatives and exhibited remarkable photo- and heat stability. Fluorescence and mass spectrometric analysis confirmed presence of a quercetin derivative. We further used this fluorophore to successfully label the silicate shell of diatom species Nitzschia palea.
Item Type: | Article |
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Source: | Copyright of this article belongs to Nature Publishing Group. |
Keywords: | Chromatography; Natural Products; Biophysical Chemistry; Biomaterials – cells |
ID Code: | 98300 |
Deposited On: | 14 May 2014 10:06 |
Last Modified: | 14 May 2014 10:06 |
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