Devaraj, K. B. ; Kumar, Parigi Ramesh ; Prakash, V. (2009) Characterization of acid-induced molten globule like state of ficin International Journal of Biological Macromolecules, 45 (3). pp. 248-254. ISSN 0141-8130
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.ijbiomac.2009.05.008
Abstract
Effect of pH on the conformational behaviour of ficin (EC 3.4.22.3), a cysteine protease from the latex of Ficus carica was monitored by circular dichroism, fluorescence spectroscopy, ANS binding and hydrodynamic studies. The results obtained from near- and far-UV CD, intrinsic fluorescence and ANS binding studies demonstrate that ficin exhibits the characteristic properties of molten globule at acidic conditions between pH 1.4 and 2.0. Ficin at pH 1.4 retained about ∼74% secondary structure with a substantial loss of tertiary structure. The acid-induced state was found to have a compact shape as measured by Stokes radius on size exclusion chromatography.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Ficin; Protein Folding; Molten Globule; Acid-Unfolding; Cysteine Protease |
| ID Code: | 97603 |
| Deposited On: | 22 May 2013 07:06 |
| Last Modified: | 22 May 2013 07:06 |
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