Komath, Sneha Sudha ; Nadimpalli, Siva Kumar ; Swamy, Musti J. (1996) Purification in high yield and characterisation of the galactose-specific lectin from the seeds of snake gourd (Trichosanthes anguina) IUBMB Life, 39 (2). pp. 243-252. ISSN 1521-6543
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Official URL: http://dx.doi.org/10.1080/15216549600201251
Related URL: http://dx.doi.org/10.1080/15216549600201251
Abstract
The galactose-specific lectin present in the seeds of snake gourd (Trichosanthes anguina) was purified in high yield by affinity chromatography on cross-linked guar gum. The purified snake gourd seed lectin (SGSL) yielded a single symmetrical peak on gel filtration with an Mr of 62 kDa and gave a single band in PAGE under non-denaturing conditions. In SDS-PAGE, SGSL gave a single band of Mr 53 kDa in the absence of β-mercaptoethanol, and two bands of Mr 32 and 23 kDa in its presence, indicating that the lectin is a heterodimer in which the subunits are linked by a disulphide bridge. The lectin gave a single precipitin line in immunodiffusion experiments with antiserum raised against the purified SGSL. No cross-reactivity was found between SGSL and antiserum raised against the Momordica charantia lectin and vice versa, suggesting that the two lectins are antigenically dissimilar. Haemagglutination-inhibition data show that MeβD-Gal is the best monosaccharide inhibitor of SGSL and indicate that an equatorial hydroxyl at C-2 and axial hydroxyl at C-4 in the pyranose form are important binding loci for the lectin.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons. |
ID Code: | 95894 |
Deposited On: | 07 Dec 2012 05:00 |
Last Modified: | 07 Dec 2012 05:00 |
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