Vashistha, Nidhi ; Arora, Taruna ; Nath, Rhisitha ; Muralidhar, Kambadur (2012) A simple method for isolation of three reproductive hormones together from the same batch of freshly frozen buffalo pituitaries collected from abattoirs Novus International Journal of Pharmaceutical Technology,, 1 (2). pp. 9-17.
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Official URL: http://novusscientia.org/?p=496
Abstract
Pseudo-affinity chromatography using Concanavaline-A-Sepharose, the immobilized form of the well known plant lectin, was used to isolate the glycoprotein fraction of buffalo pituitary extract. The lectin bound proteins were eluted and stored lyophilized. This isolate was analyzed by ELISA and by hormone specific bioassays in rats to obtain potency estimates of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and Thyroid Stimulating Hormone (TSH) content. Hopefully this isolate will be useful in inducing super ovulation in animals including buffaloes.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Novus Scientia Journals. |
| Keywords: | Pseudo-affinity Chromatography; Concanavaline-A-Sepharose; ELISA |
| ID Code: | 95836 |
| Deposited On: | 23 Nov 2012 12:11 |
| Last Modified: | 23 Nov 2012 12:11 |
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