Lack of association of single nucleotide polymorphisms in toll-like receptors 2 and 4 with enthesitis-related arthritis category of juvenile idiopathic arthritis in Indian population

Abstract

Toll-like receptors 2 and 4 are over expressed in patients with enthesitis-related arthritis and cause increased production of pro-inflammatory cytokines. This aberrant functioning could be due to polymorphisms in TLR2 and TLR4. Hence, we genotyped ERA patients for Arg753Gln and Arg677Trp polymorphism in TLR2 gene and Asp299Gly and Thr399Ile polymorphism in TLR4 gene. DNA was extracted from blood from ERA patients and healthy controls. All four polymorphisms were studied by PCR–RFLP method. 200 healthy controls and 97 ERA patients were enrolled. All healthy controls and patients had wild-type allele for Arg753Gln and Arg677Trp TLR2 polymorphism. Regarding TLR4, Asp299Gly polymorphism A allele frequency was 90 % in controls and 96 % in patients (OR 2.7, 95 % CI 0.81–8.8). GG homozygous genotype was detected in one healthy control and was absent from patients. The TLR4 Thr399Ileu variant was not detected in patients. Out of 200 healthy controls, 10 were heterozygous (5 %) and only one was homozygous for rare variant (0.5 %). Polymorphisms in TLR2 and TLR4 are not associated with ERA.