⁽¹⁾H, ⁽¹³⁾C, ⁽¹⁵⁾N assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii

Abstract

Sequence specific resonance assignments have been obtained for ¹H, ¹³C and ¹⁵N nuclei of the 21 kDa (188 residues long) glutamine amido transferase subunit of guanosine monophosphate synthetase from Methanocaldococcus jannaschii. From an analysis of ¹H and ¹³C^α, ¹³C^β secondary chemical shifts, ³ JH^NH^α scalar coupling constants and sequential, short and medium range ¹H–¹H NOEs, it was deduced that the glutamine amido transferase subunit has eleven strands and five helices as the major secondary structural elements in its tertiary structure.