Proteophosphoglycan is differentially expressed in sodium stibogluconate-sensitive and resistant Indian clinical isolates of Leishmania donovani

Samant, M. ; Sahasrabuddhe, A. A. ; Singh, N. ; Gupta, S. K. ; Sundar, S. ; Dube, A. (2007) Proteophosphoglycan is differentially expressed in sodium stibogluconate-sensitive and resistant Indian clinical isolates of Leishmania donovani Parasitology, 134 (9). pp. 1175-1184. ISSN 0031-1820

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Related URL: http://dx.doi.org/10.1017/S0031182007002569

Abstract

Leishmania produce several types of mucin-like glycoproteins called proteophosphoglycans (PPGs) some of which are secreted while others are found on the surface of promastigotes and amastigotes. These proteins are thought to be important in the transmission, invasion and subsequent intracellular survival of parasites. The structure and function of PPGs are species and stage-specific in the case of L. major and L. mexicana, but no such information has hitherto been available for L. donovani. This study presents, for the first time, an initial characterization (localization) of PPG in sodium stibogluconate (SSG)-resistant and sensitive clinical isolates of L. donovani from Bihar (India) by confocal microscopy, flow cytometry and Western blotting using antibodies to L. major PPG. Confocal microscopy analysis revealed that both promastigotes and amastigotes possess PPG on their cell membrane and flagellar pocket membrane but its expression was variable in different isolates. The quantitative analysis by FACS and Western blotting showed that the expression and intensity of PPG bands was higher in SSG-resistant isolates. This study suggests the possibilities of involvement of PPG in drug-resistant mechanisms and of using PPG abundance as a marker for identifying drug-resistant clinical isolates in Indian kala azar.

Item Type:Article
Source:Copyright of this article belongs to Cambridge University Press.
Keywords:Proteophosphoglycans; Leishmania Donovani; SSG-Sensitive and Resistant Clinical Isolates; Confocal Microscopy; Flow Cytometry; Western Blotting
ID Code:94543
Deposited On:16 Nov 2012 12:09
Last Modified:27 Oct 2015 11:40

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