Gupta, Reema ; Kushawaha, Pramod K. ; Tripathi, Chandra Dev Pati ; Sundar, Shyam ; Dube, Anuradha (2012) A novel recombinant Leishmania donovani p45, a partial coding region of methionine aminopeptidase, generates protective immunity by inducing a Th1 stimulatory response against experimental visceral Leishmaniasis International Journal for Parasitology, 42 (5). pp. 429-435. ISSN 0020-7519
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.ijpara.2012.02.013
Abstract
The development of a vaccine against visceral leishmaniasis (VL) conferring long-lasting immunity remains a challenge. Identification and proteomic characterization of parasite proteins led to the detection of p45, a member of the methionine aminopeptidase family. To our knowledge the present study is the first known report that describes the molecular and immunological characterization of p45. Recombinant Leishmania donovani p45 (rLdp45) induced cellular responses in cured hamsters and generated Th1-type cytokines from peripheral blood mononuclear cells of cured/endemic VL patients. Immunization with rLdp45 exerted considerable prophylactic efficacy (∼85%) supported by an increase in mRNA expression of iNOS, IFN-γ, TNF-α and IL-12 and decrease in TGF-β and IL-4, indicating its potential as a vaccine candidate against VL.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Leishmania Donovani; Recombinant p45; Methionine Aminopeptidase; Immunogenicity; T-Cell Response; Cured Leishmania Hamsters; Cured Leishmania Patients And Endemic Contacts; Prophylactic Potential |
| ID Code: | 94396 |
| Deposited On: | 15 Nov 2012 06:55 |
| Last Modified: | 27 Oct 2015 11:34 |
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