Isoenzyme patterns of several hydrolytic enzymes of Agrobacterium tumefaciens

Abstract

The isoenzyme patterns of several hydrolytic enzymes, viz., acid phosphatase, alkaline phosphatase, and catalase, in virulent and avirulent, agrocin-resistant Agrobacterium tumefaciens have been studied. Agrocin resistance brought about genetic repression in regard to the synthesis of one of the isoenzymes in each of these three enzymes. Avirulent resistant strains also showed a reduction in the activities of all the hydrolases. Although Mg²⁺ stimulated the activity of alkaline phosphatase, no absolute requirement of any metal ions for the activity of the enzyme was observed.