S-layer homology motif is an immunogen and confers protection to mouse model against anthrax

Abstract

SLH proteins bear an S-layer homology motif comprised of three S-layer homology (SLH) domains. Several SLH proteins in Bacillus anthracis have been recognized as immunogenic in recent past. We hypothesized that the SLH motif, the most common moiety amongst all the SLH proteins could be responsible for their immunogenicity. To test this hypothesis, we checked the immunogenic capacity of recombinant SLH motif. The rSLH fragment on immunization in mice led to the development of a potent humoral and T Helper immune response as compared to the only adjuvant immunized group. Antibodies raised against rSLH could identify the surface of B. anthracis Ames strain vegetative forms. rSLH immunization protected 50% mice challenged with B. anthracis compared to 0% survival in group of mice immunized with only adjuvant. But when rSLH immunization was synergized with a single sub-optimal dose of rPA (Protective Antigen), 80% immunized mice survived the lethal challenge of B. anthracis. Taken together, for the first time we demonstrate the immunogenic and protective potential of SLH motif of the SLH proteins of B. anthracis.