Characterization of amino acid-derived betaines by electrospray ionization tandem mass spectrometry

Chary, Naresh V. ; Kumar, Dinesh Ch. ; Vairamani, M. ; Prabhakar, S. (2012) Characterization of amino acid-derived betaines by electrospray ionization tandem mass spectrometry Journal of Mass Spectrometry, 47 (1). pp. 79-88. ISSN 1076-5174

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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/jms.202...

Related URL: http://dx.doi.org/10.1002/jms.2029

Abstract

Betaines belong to the naturally occurring osmoprotectants or compatible solutes present in a variety of plants, animals and microorganisms. In recent years, metabolomic techniques have been emerging as a fundamental tool for biologists because the constellation of these molecules and their relative proportions provide with information about the actual biochemical condition of a biological system. Therefore, identification and characterization of biologically important betaines are crucial, especially for metabolomic studies. Most of the natural betaines are derived from amino acids and related homologues. Although, theoretically, all the amino acids can be converted to corresponding betaines by simple methylation of the amine group, only a few of the amino acid-derived betaines were fully characterized in the literature. Here, we report a combined electrospray ionization tandem and high-resolution mass spectrometry study of all the betaines derived from amino acids, including the isomeric betaines. The decomposition pathway of protonated, sodiated and potassiated molecule ions that enable unambiguous characterization of the betaines including the isomeric betaines and overlapping ionic species of different betaines is distinctive.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Betaines; Electrospray Ionization; Protonated and Cationized Betaines; Tandem Mass Spectrometry; High-resolution Mass Spectrometry
ID Code:92762
Deposited On:04 Jun 2012 06:27
Last Modified:04 Jun 2012 06:27

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