Tyagi, R. K. ; Datta, K. (1994) A rapid, high-yield purification of L-alanine: 4,5-dioxovalerate transaminase from rat kidney mitochondria using an improved enzyme assay method Protein Expression and Purification, 5 (6). pp. 527-533. ISSN 1046-5928
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S10465...
Related URL: http://dx.doi.org/10.1006/prep.1994.1072
Abstract
The present report documents an improved enzyme assay method for the mammalian L-alanine:4,5-dioxovalerate transaminase which is of significant utility in work with crude tissue homogenates, cell cultures, or purified enzyme preparations. We also describe a new and rapid purification procedure for this enzyme from rat kidney mitochondria. The three-step procedure involves the use of digitonin and lubrol for mitochondrial matrix preparation and L-alanine-Sepharose 4B column chromatography followed by gel filtration on Sepharose 6B. By this procedure it is possible to obtain a highly purified enzyme preparation in a relatively short time with a 37.5% yield.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 9104 |
| Deposited On: | 29 Oct 2010 11:36 |
| Last Modified: | 28 May 2011 10:49 |
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