Mukerjee, R. ; Chaturvedi, U. C. (1997) ELISA for detection of dengue virus-induced cytokine and its antibody Indian Journal of Experimental Biology, 35 (3). pp. 225-231. ISSN 0019-5189
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Abstract
ELISA technique has been standardized for detection of a dengue type 2 virus (DV)-induced cytokine, the cytotoxic factor (CF) and CF-specific antibodies. The performed ELISA was found to be sensitive (90.9%), specific (92.5%), accurate (91%) and reproducible and was able to detect a minimum of 7 ng/ml CF in the test samples. The technique was used to detect CF in DV inoculated mouse sera and DV-infected mouse spleen cell culture fluids. Significant utility of the test was detection of a CF-like cytokine in the sera of human cases of dengue haemorrhagic fever.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to National Institute of Science Communication and Information Resources. |
| ID Code: | 90524 |
| Deposited On: | 10 May 2012 10:36 |
| Last Modified: | 10 May 2012 10:36 |
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