Control of Salmonella in frog legs by chemical and physical methods

Abstract

Hexamine was quite effective at 0.025%(wt/vol) in nutrient broth against Salmonella. But a 3% conc. of it was required to destroy. Salmonella in frog legs. However, a synergistic effect was observed between NaCl(10%) and hexamine (0.25%) when tested against Salmonella contaminated frog legs. pH imparted considerable influence on the viability of salmonella and on the concentration of antiseptics used. Ethylene oxide fumigation was effective in sterilizing the contaminated frog legs. Salmonella growth was also inhibited by heat treatment given in different phases. UV irradiation on Salmonella contaminated frog legs did not produce any significant effect, while irradiation produced promising results. Cent percent destruction was observed at 0.5 and 0.7 M rad when the initial counts of salmonella were 2.5 ×10⁵ and 3.6×10⁵/g of frog legs.