Das, Biswadip ; Chattopadhyay, Subrata ; Das Gupta, Chanchal (1992) Reactivation of denatured fungal glucose 6-phosphate dehydrogenase and E.coli alkaline phosphatase with E.coli ribosome Biochemical and Biophysical Research Communications, 183 (2). pp. 774-780. ISSN 0006-291X
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...
Related URL: http://dx.doi.org/10.1016/0006-291X(92)90550-5
Abstract
Fungal glucose 6-phosphate dehydrogenase and E.coli alkaline phosphatase were denatured either by physical or by chemical means. In vitro reconstitution of these denatured enzymes was assisted by 70S E.coli ribosome, as shown by the recovery of their catalytic competence. Almost total recovery of the activities of completely inactivated enzymes was obtained when 70S ribosome was present at about equimolar concentration with the enzyme molecules at 37C and 50C, respectively.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 8882 |
| Deposited On: | 28 Oct 2010 08:49 |
| Last Modified: | 28 May 2011 09:21 |
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