The isolation of two facultatively Anaerobic organisms producing collegenase

Abstract

One Bacillus sp. and one psedomonas sp. isolated from putrefied skin were found to secrete extracellular collagenases under both aerobic and anaerobic conditions of growth. Collegenase activity was determined by studying the hydrolysis of RTT collagen fibers, lysis of collagen gel and reduction in viscosity of soluble collagen. In all the tests collagenolytic activity of these two isolates was compared with that of Clostridium histolyticum. Protease elaborated by the facultatively anaerobic organisms also hydrolysed caesin, egg albumin and gelatin.