Skin hydrolysis by two newly isolated tatively anaerobic orgainsms

Abstract

Two facultatively anaerobic organisms (one Bacillus sp. and one Pseudomonas sp.) which were earlier found to possess collagenolytic activity have been examined more closely for their hydrolytic action on skin collagen. Bacillus sp, appeared to possess strong hydrolytic action whereas Pseudomonas sp. is much weaker in its action at 37°C under' both aerobic and anaerobic conditions. Collagen hydrolysis, by both these organisms is appreciably retarded above 5% NaCl concentration. Pseudomonas sp, is more sensitive to collagenase' inhibitors than the Bacillus sp. The optimum pH and temperature for skin hydrolysis by both the organisms have been fouod out. UV irradiation of the inoculum affects tbe collagenolytic activity of the Bacillus sp. to a considerable extent. Both the organisms are capable of unhairing the skin completely within 3 days. Bacillus sp is responsible for skin degradation to a greater extent compared to that of Pseudomonas sp.