Ehtesham, Nasreen Z. ; Hasnain, Seyed E. (1991) Direct in-gel hybridization, without blotting, using nick-translated cloned DNA probe Biotechniques, 11 (6). pp. 718-722. ISSN 0736-6205
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Official URL: http://www.biotechniques.com/BiotechniquesJournal/...
Abstract
Hybridization of DNA and RNA to labeled probes is central to molecular biology. A method is described here for hybridization of cloned DNA probes directly to DNA in agarose gels. This in-gel hybridization method has several advantages over conventional techniques using transfer to membranes. It is extremely rapid, highly sensitive, less expensive and particularly suited for high molecular weight genomic DNA analysis.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Informa plc. |
| ID Code: | 88623 |
| Deposited On: | 29 Mar 2012 09:26 |
| Last Modified: | 29 Mar 2012 09:26 |
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