Tundup, Smanla ; Pathak, Niteen ; Ramanadham, M. ; Mukhopadhyay, Sangita ; Murthy, K. J. R. ; Ehtesham, Nasreen Z. ; Hasnain, Seyed E. (2008) The co-operonic PE25/PPE41 protein complex of Mycobacterium tuberculosis elicits increased humoral and cell mediated immune response Plos One, 3 (10). e3586_1-e3586_9. ISSN 1932-6203
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Official URL: http://www.plosone.org/article/info:doi%2F10.1371%...
Related URL: http://dx.doi.org/10.1371/journal.pone.0003586
Abstract
Background: Many of the PE/PPE proteins are either surface localized or secreted outside and are thought to be a source of antigenic variation in the host. The exact role of these proteins are still elusive. We previously reported that the PPE41 protein induces high B cell response in TB patients. The PE/PPE genes are not randomly distributed in the genome but are organized as operons and the operon containing PE25 and PPE41 genes co-transcribe and their products interact with each other. Methodology/Principal Finding: We now describe the antigenic properties of the PE25, PPE41 and PE25/PPE41 protein complex coded by a single operon. The PPE41 and PE25/PPE41 protein complex induces significant (p<0.0001) B cell response in sera derived from TB patients and in mouse model as compared to the PE25 protein. Further, mice immunized with the PE25/PPE41 complex and PPE41 proteins showed significant (p<0.00001) proliferation of splenocyte as compared to the mice immunized with the PE25 protein and saline. Flow cytometric analysis showed 15-22% enhancement of CD8+ and CD4+ T cell populations when immunized with the PPE41 or PE25/PPE41 complex as compared to a marginal increase (8-10%) in the mice immunized with the PE25 protein. The PPE41 and PE25/PPE41 complex can also induce higher levels of IFN-γ, TNF-α and IL-2 cytokines. Conclusion: While this study documents the differential immunological response to the complex of PE and PPE vis-à-vis the individual proteins, it also highlights their potential as a candidate vaccine against tuberculosis.
Item Type: | Article |
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Source: | Copyright of this article belongs to Public Library of Science. |
ID Code: | 88583 |
Deposited On: | 29 Mar 2012 09:43 |
Last Modified: | 19 May 2016 03:24 |
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