Sadashiva Karnik, S. ; Gopinathan, K. P. (1981) Presence of nucleoside triphosphates and calcium associated with mycobacteriophage I3 Archives of Microbiology, 130 (1). pp. 50-53. ISSN 0302-8933
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Official URL: http://www.springerlink.com/content/m5763642464341...
Related URL: http://dx.doi.org/10.1007/BF00527071
Abstract
The association of nucleoside triphosphate molecules and calcium ions with purified particles of mycobacteriophage I3 has been documented. The content of nucleoside triphosphate has been determined to be 118 molecules per phage particle by equilibrium dialysis against labelled ATP or 148 molecules per phage particle by the direct determination of labelled nucleoside triphosphate. The concentration of bound Ca2+ exhibited a high degree of variation between different batches, which may be due to the nonspecific binding of Ca2+ by the virus particles. However, the tightly bound Ca2+ not removable by dialysis against calciumspecific chelating agent, showed a constant value of 2985 atoms/phage particle.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| Keywords: | Bound NTPs; Injection Of DNA; Phage Bound Ca2+; Equilibrium Dialysis; Mycobacteriophage I3 |
| ID Code: | 88085 |
| Deposited On: | 27 Mar 2012 08:06 |
| Last Modified: | 27 Mar 2012 08:06 |
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