Natarajan, V. ; Gopinathan, K. P. (1981) Mechanism of aminoacylation of tRNA: influence of spermine on the kinetics of aminoacyl-tRNA synthesis by isoleucyl- and valyl-tRNA synthetases from Mycobacterium smegmatis Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 654 (1). pp. 94-101. ISSN 0005-2787
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0005-2787(81)90140-4
Abstract
Isoleucyl-tRNA synthetase has been purified to homogeneity from Mycobacterium smegmatis. The influence of spermine on the kinetics of valyl-tRNA and isoleucyl-tRNA formation has been investigated by Cleland's method (Cleland, W.W. (1963) Biochim. Biophys. Acta 67, 104-137, 173-187, 188-196). The results suggest that in the presence of spermine and suboptimal concentration of Mg2+, the formation of valyl-tRNA and isoleucyl-tRNA follows a sequential mechanism. In the presence of an optimal concentration of Mg2+, both valyl-tRNA and isoleucyl-tRNA formation proceeds by a ping-pong mechanism. However, in the presence of spermine and optimal concentrations of Mg2+, valyl-tRNA formation follows the ping-pong mechanism while isoleucyl-tRNA formation follows the sequential mechanism.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Aminoacylation; tRNA; Spermine; Isoleucyl-tRNA Synthetase; Valyl-tRNA Synthetase; (M. Smegmatis) |
| ID Code: | 88084 |
| Deposited On: | 27 Mar 2012 08:16 |
| Last Modified: | 27 Mar 2012 08:16 |
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