Salomon, Robert G. ; Jirousek, Michael R. ; Ghosh, Subrata ; Sharma, Ram B. (1987) Prostaglandin endoperoxidase. 21. Covalent binding of levuglandin E2 with proteins Prostaglandins & Other Lipid Mediators, 34 (5). pp. 643-656. ISSN 0090-6980
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0090-6980(87)90289-9
Abstract
Levuglandin E2(LGE2), a γ -ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, binds covalently with ram seminal vesicle microsomes. Totally synthesis 5,6-ditritio-LGE2 was prepared and used to determine that rapid covalent binding of LGE2 (initially 800 μ M) occurs with 6.4 μ M bovine serum albumin (>10 equiv within 1 min) which approached saturation (≈ 16 equiv) after 40 min at 37° C.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 88016 |
| Deposited On: | 26 Mar 2012 13:52 |
| Last Modified: | 26 Mar 2012 13:52 |
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