Iyer, R. S. ; Ghosh, S. ; Salomon, R. G. (1989) Levuglandin E2 crosslinks proteins Prostaglandins & Other Lipid Mediators, 37 (4). pp. 471-480. ISSN 0090-6980
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0090-6980(89)90096-8
Abstract
Levuglandin E2 (LGE2), a γ -ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, causes extensive intermolecular crosslinking of ovalbumin at pH 6 or pH 7 and 37° C. The time dependence of protein oligomerization is monitored by SDS-PAGE. Effects of pH and concentration on the extent of LGE2-induced crosslinking are examined. The efficacy of LGE2 for inducing crosslinking is compared with other oxidative metabolites of arachidonic acid (AA), including the prostaglandins PGE2, PGD2, PGA2, PGB2, and PGF2α , as well as malondialdehyde and E-4-hydroxy-non-2-enal. LGE2 is orders of magnitude more effective in crosslinking protein than any other cyclooxygenase or lipoxygenase metabolite of AA tested.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
ID Code: | 88015 |
Deposited On: | 26 Mar 2012 13:52 |
Last Modified: | 26 Mar 2012 13:52 |
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