Hull, R. ; Jones, M. C. ; Dasgupta, I. ; Cliffe, J. M. ; Mingins, C. ; Lee, G. ; Davies, J. W. (1990) Molecular biology of rice tungro viruses: evidence for a new retroid virus Rice Genetics II, 19th Stadler Genetics Symposium . pp. 539-547.
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Official URL: http://eproceedings.worldscinet.com/9789812814272/...
Related URL: http://dx.doi.org/10.1142/9789812814272_0055
Abstract
Rice tungro spherical virus (RTSV) particles contain a single-stranded RNA genome with a major species of about 10 kb. RTSV particles have several species of coat protein. Two major proteins (35.5 and 25 kDa) were detected in protein extracts from infected leaves that were probed on western blots with a polyclonal antiserum raised against purified virus: a third protein (29 kDa) was found after Coomassie Brilliant Blue staining of gels. Two species of coat protein (36 and 32 kDa) were detected in rice tungro bacilliform virus preparations by western blotting. The particles contain double-stranded DNA of 8.3 kbp that has structural features (different conformations and singlestrand discontinuities) resembling those of caulimovirus DNA. Preliminary sequence data also revealed a primer binding site and a putative promoter sequence similar to those of cauliflower mosaic virus DNA.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Rice Genetics II, 19th Stadler Genetics Symposium. |
| ID Code: | 86673 |
| Deposited On: | 12 Mar 2012 13:54 |
| Last Modified: | 12 Mar 2012 14:03 |
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