Agrewala, Javed N. ; Raghava, G. P. S. ; Mishra, Gyan C. (1993) Measurement and computation of murine interleukin-4 and interferon-γ by exploiting the unique abilities of these lymphokines to induce the secretion of IgG1 and IgG2a Journal of Immunoassay, 14 (1-2). pp. 83-97. ISSN 0197-1522
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Official URL: http://www.tandfonline.com/doi/abs/10.1080/1532181...
Related URL: http://dx.doi.org/10.1080/15321819308019842
Abstract
A specific and new method for measuring Interleukin-4 and Interferon-γ, based on the estimation of IgG1 and IgG2a isotypes secretion from B cells is described. An antagonizing effect of Interferon-? in the production of IgG1 induced by Interleukin-4 was neutralized by using antibody to Interferon-γ. Similarly, the interference of Interleukin-4 in the Interferon-γ mediated enhancement of IgG2a production was blocked by anti-Interleukin-4 antibody. The high concentrations of Interleukin-4 and Interferon γ inhibited the secretion of IgG1 and IgG2a respectively. Therefore, in the assay described, the samples containing the cytokines were so diluted that their activity fell into the non-inhibitory zone. A computer program has also been developed for determining the concentrations of lymphokines
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor and Francis Group. |
| Keywords: | Interleukin-4; Interferon-γ Computer Program; Immunoglobulin Isotype |
| ID Code: | 86645 |
| Deposited On: | 12 Mar 2012 13:49 |
| Last Modified: | 04 Jul 2012 12:36 |
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