Ray, Partho Sarothi ; Grover, Richa ; Das, Saumitra (2006) Two internal ribosome entry sites mediate the translation of p53 isoforms EMBO reports, 11 (7). 404- 410. ISSN 1469-221X
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Official URL: http://www.nature.com/embor/journal/vaop/ncurrent/...
Related URL: http://dx.doi.org/10.1038/sj.embor.7400623
Abstract
The p53 tumour suppressor protein has a crucial role in cell-cycle arrest and apoptosis. Previous reports show that the p53 messenger RNA is translated to produce an amino-terminal-deleted isoform (ΔN-p53) from an internal initiation codon, which acts as a dominant-negative inhibitor of full-length p53. Here, we show that two internal ribosome entry sites (IRESs) mediate the translation of both full-length and ΔN-p53 isoforms. The IRES directing the translation of full-length p53 is in the 5'-untranslated region of the mRNA, whereas the IRES mediating the translation of ΔN-p53 extends into the protein-coding region. The two IRESs show distinct cell-cycle phase-dependent activity, with the IRES for full-length p53 being active at the G2-M transition and the IRES for ΔN-p53 showing highest activity at the G1-S transition. These results indicate a novel translational control of p53 gene expression and activity.
Item Type: | Article |
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Source: | Copyright of this article belongs to European Molecular Biology Organization. |
Keywords: | IRES; P53; Translation; Cell cycle |
ID Code: | 8622 |
Deposited On: | 28 Oct 2010 11:16 |
Last Modified: | 16 May 2016 18:34 |
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