Vaidya, Bhalchandra K. ; Ingavle, Ganesh C. ; Ponrathnam, S. ; Kulkarni, B. D. ; Nene, Sanjay N. (2008) Immobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous polymer particles Bioresource Technology, 99 (9). pp. 3623-3629. ISSN 0960-8524
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.biortech.2007.07.035
Abstract
Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Epoxy-activated Support; Enzyme Immobilization; Candida rugosa Lipase |
| ID Code: | 85704 |
| Deposited On: | 05 Mar 2012 14:05 |
| Last Modified: | 05 Mar 2012 14:05 |
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