Katta, Santharam S. ; Satish Tammana, Trinadh V. ; Sahasrabuddhe, Amogh A. ; Bajpai, Virendra K. ; Gupta, Chhitar M. (2010) Trafficking activity of myosin XXI is required in assembly of Leishmania flagellum Journal of Cell Science, 123 . pp. 2035-2044. ISSN 0021-9533
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Official URL: http://jcs.biologists.org/content/123/12/2035.shor...
Related URL: http://dx.doi.org/10.1242/jcs.064725
Abstract
Actin-based myosin motors have a pivotal role in intracellular trafficking in eukaryotic cells. The parasitic protozoan organism Leishmania expresses a novel class of myosin, myosin XXI (Myo21), which is preferentially localized at the proximal region of the flagellum. However, its function in this organism remains largely unknown. Here, we show that Myo21 interacts with actin, and its expression is dependent of the growth stage. We further reveal that depletion of Myo21 levels results in impairment of the flagellar assembly and intracellular trafficking. These defects are, however, reversed by episomal complementation. Additionally, it is shown that deletion of the Myo21 gene leads to generation of ploidy, suggesting an essential role of Myo21 in survival of Leishmania cells. Together, these results indicate that actin-dependent trafficking activity of Myo21 is essentially required during assembly of the Leishmania flagellum.
Item Type: | Article |
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Source: | Copyright of this article belongs to The Company of Biologists Ltd. |
Keywords: | Trypanosomatids; Intraflagellar Transport; Myosin; Trafficking |
ID Code: | 85630 |
Deposited On: | 05 Mar 2012 06:44 |
Last Modified: | 19 May 2016 01:35 |
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