Identification of amino acid residues at the active site of human liver serine hydroxymethyltransferase

Vijayalakshmi, D. ; Rao, N. A. (1989) Identification of amino acid residues at the active site of human liver serine hydroxymethyltransferase Biochemistry International, 19 (3). pp. 625-632. ISSN 0158-5231

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Abstract

Chemical modification of amino acid residues with phenylglyoxal, diethylpyrocarbonate, and N-bromosuccinimide indicated that at least one residue each of arginine, histidine, and tryptophan were necessary for the activity of human liver serine hydroxymethyltransferase. Protection by substrates suggested that these residues might occur at the active site of the enzyme.

Item Type:Article
Source:Copyright of this article belongs to International Union of Biochemistry by Academic Press.
ID Code:85228
Deposited On:01 Mar 2012 09:19
Last Modified:01 Mar 2012 09:19

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