Enhancement of androgenesis by abiotic stress and other pretreatments in major crop species

Abstract

Rapid production of doubled haploids (DHs) through androgenesis is an important and promising method for genetic improvement of crop plants. Through androgenesis complete homozygous plants can be produced within a year compared to long inbreeding methods that may take several years and costly. Significant advantage of androgenesis is that it not only speeds up the process to achieve homozygosity, but also increases the selection efficiency. Though success in androgenesis has been achieved in many crop plants, yet there are certain limitations especially, low frequency of embryogenesis and regeneration in few species. In fact in many cereals, induction of embryos and regeneration of green plants is still a hurdle that one needs to overcome to improve the efficiency of androgenesis. Efficient androgenesis is usually induced by the successful application of different stress pretreatment. Since so many stress factors can trigger the reprogramming of microspores and that have been co-related to change the ultrastuctural changes of cells to embryos and finally haploid plants. It has been shown that certain pretreatment such as (i) physical stresses as cold, heat shock, starvation, drought stress, osmotic pressure, gamma irradiation, oxidative stress, reduced atmospheric pressure, and (ii) chemical treatments such as colchicine, heavy metal, ABA, CGA, AEC, Azetidine, 2-NHA, either individual or combined effect of more than one stress factors may positively influence androgenetic efficiency. This review highlights the recent and past work on uses of various abiotic stresses and pretreatments and their impact on enhancing the efficiency of androgenesis on some major crop species for the development of doubled haploid plants.