Shiga-toxin producing Escherichia coli from healthy cattle in a semi-Urban community in Calcutta, India

Pal, A. ; Ghosh, S. ; Ramamurthy, T. ; Yamasaki, S. ; Tsukamoto, T. ; Bhattacharya, S. K. ; Nair, G. B. ; Takeda, Y. (1999) Shiga-toxin producing Escherichia coli from healthy cattle in a semi-Urban community in Calcutta, India Indian Journal of Medical Research, 110 . pp. 83-85. ISSN 0971-5916

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Abstract

From October to December 1998, single faecal samples from 67 healthy cattle in a semi-urban community near Calcutta were examined for Shiga toxin producing Esch. coli (STEC) using a multiplex PCR primary screen followed by plating on sorbitol MacConkey agar. STEC was isolated from the faeces of 7 (10.5%) animals. The eight strains isolated belonged to eight serotypes viz, O146:H1, O149:HNT, ONT:H34, ONT:H19, O88:HN, ONT:H2, O82:H8 and O28ac:H21. Bead enzyme linked immunosorbent assay showed that three strains produced Shiga toxin 1, one produced Shiga toxin 2 and four produced both.

Item Type:Article
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