Studies of J-connectivities and selective 1H-1H Overhauser effects in H2O solutions of biological macromolecules by two-dimensional NMR experiments

Anil Kumar, ; Wagner, G. ; Ernst, R. R. ; Wuthrich, K. (1980) Studies of J-connectivities and selective 1H-1H Overhauser effects in H2O solutions of biological macromolecules by two-dimensional NMR experiments Biochemical and Biophysical Research Communications, 96 (3). pp. 1156-1163. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...

Related URL: http://dx.doi.org/10.1016/0006-291X(80)90073-X

Abstract

This paper demonstrates that two-dimensional (2D) NMR experiments are particularly suitable for studies of biopolymers in H2O solution. Compared to conventional one-dimensional NMR experiments the 2D studies are much more efficient and yield with a single instrument setting a nearly complete network of J-connectivities or cross relaxation pathways involving labile protons. The use of these techniques in H2O solutions presents a new approach for detailed studies of the backbone spatial structure in proteins and should further be of particular interest for conformational studies of nucleic acids. Here, homonuclear J-connectivities and Overhauser effects (NOE) involving labile protons were studied in the basic pancreatic trypsin inhibitor.

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