Mitra, Tapas ; Sailakshmi, G. ; Gnanamani, A. ; Thirupathi Kumara Raja, S. ; Thiruselvi, T. ; Mangala Gowri, V. ; Selvaraj, Naga Vignesh ; Ramesh, Gopal ; Mandal, A. B. (2011) Preparation and characterization of a thermostable and biodegradable biopolymers using natural cross-linker International Journal of Biological Macromolecules, 48 (2). pp. 276-285. ISSN 0141-8130
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.ijbiomac.2010.11.011
Abstract
The present study describes preparation and characterization of a thermally stable and biodegradable biopolymer using collagen and a natural polymer, alginic acid (AA). Required concentration of alginic acid and collagen was optimized and the resulting biopolymer was characterized for, degree of cross-linking, mechanical strength, thermal stability, biocompatibility (toxicity) and biodegradability. Results reveal, the degree of cross-linking of alginic acid (at 1.5% concentration) with collagen was calculated as 75%, whereas it was 83% with standard cross-linking agent, glutaraldehyde (at 1.5% concentration). The AA cross-linked biopolymer was stable up to 245°C and Exhibits 5-6-fold increase in mechanical (tensile) strength compared to plain collagen (native) materials. However, glutaraldehyde cross-linked material exhibits comparatively less thermal stability and brittle in nature (low tensile strength). With regard to cell toxicity, no cytotoxicity was observed for AA cross-linked material when tested with mesenchymal cells and found degradable when treated with collagenase enzyme. The nature of bonding pattern and the reason for thermal stability of AA cross-linked collagen biopolymer was discussed in detail with the help of bioinformatics. A supplementary file on efficacy of AACC as a wound dressing material is demonstrated in detail with animal model studies.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Type I Collagen; Alginic Acid; Biopolymer; Biocompatibility; Biodegradability; Thermal Stability |
ID Code: | 83854 |
Deposited On: | 23 Feb 2012 07:16 |
Last Modified: | 23 Feb 2012 07:16 |
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