Intergeneric somatic hybrids of rice [Oryza sativa L.(+) Porteresia coarctata (Roxb.) Tateoka]

Jelodar, N. B. ; Blackhall, N. W. ; Hartman, T. P. V. ; Brar, D. S. ; Khush, G. ; Davey, M. R. ; Cocking, E. C. ; Power, J. B. (1999) Intergeneric somatic hybrids of rice [Oryza sativa L.(+) Porteresia coarctata (Roxb.) Tateoka] Theoretical and Applied Genetics (TAG), 99 (3-4). pp. 570-577. ISSN 0040-5752

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Somatic hybrid plants were obtained following the electrofusion of rice (Oryza sativa L. cv 'Taipei 309', 2n = 2x = 24) cell suspension-derived protoplasts with non-dividing leaf protoplasts of Porteresia coarctata (2n = 4x = 48), a saline-tolerant wild species. Fusion-treated protoplasts were plated on the surface of cellulose nitrate filter membranes, overlaying Lolium multiflorum nurse cells. The nurse cells were embedded in KPR medium containing 0.5 mg l-1 2,4-dichlorophenoxyacetic acid and semi-solidified with SeaPlaque agarose. Putative somatic hybrid cell colonies were selected on the basis of their growth, whereby faster growing colonies were transferred preferentially to MS-based medium with 2.0 mg l-1 kinetin, 0.5 mg l-1 α-naphthaleneacetic acid, 30 g l-1 sucrose and 4.0 g l-1 SeaKem agarose to induce shoot regeneration. One hundred and nineteen regenerated plants were micropropagated clonally on MS-based medium containing 2.0 mg l-1 6-benzylaminopurine, 50 g l-1 sucrose and 4.0 g l-1 SeaKem agarose, prior to DNA extraction of plant samples. Putative somatic hybrids were initially identified by RAPD analysis, and 8 plant lines were selected for further investigation by flow cytometric ploidy determination and cytology. Plants of one line had an allohexaploid chromosome complement (2n = 6x = 72) and, following examination of its vegetative clones by GISH, were confirmed as somatic hybrids containing full chromosome complements of both O. sativa and P. coarctata.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Rice; Oryza sativa; Porteresia coarctata; Somatic Hybridisation; Flow Cytometry; Random Amplification of Polymorphic DNA (RAPD); Genome in situ Hybridisation (GISH); Halophyte; Chromosomes
ID Code:83815
Deposited On:23 Feb 2012 04:12
Last Modified:23 Feb 2012 04:12

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