Crystal structure of the chromodomain helicase DNA-binding protein 1 (Chd1) DNA-binding domain in complex with DNA

Sharma, Amit ; Jenkins, Katherine R. ; Hroux, Annie ; Bowman, Gregory D. (2011) Crystal structure of the chromodomain helicase DNA-binding protein 1 (Chd1) DNA-binding domain in complex with DNA Journal of Biological Chemistry, 286 . pp. 42099-42104. ISSN 0021-9258

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Official URL: http://www.jbc.org/content/286/49/42099.abstract?s...

Related URL: http://dx.doi.org/10.1074/jbc.C111.294462

Abstract

Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 chromatin remodeler possesses a C-terminal DNA-binding domain that is required for efficient nucleosome sliding and believed to be essential for sensing the length of DNA flanking the nucleosome core. The structure of the Chd1 DNA-binding domain was recently shown to consist of a SANT and SLIDE domain, analogous to the DNA-binding domain of the ISWI family, yet the details of how Chd1 recognized DNA were not known. Here we present the crystal structure of the Saccharomyces cerevisiae Chd1 DNA-binding domain in complex with a DNA duplex. The bound DNA duplex is straight, consistent with the preference exhibited by the Chd1 DNA-binding domain for extranucleosomal DNA. Comparison of this structure with the recently solved ISW1a DNA-binding domain bound to DNA reveals that DNA lays across each protein at a distinct angle, yet contacts similar surfaces on the SANT and SLIDE domains. In contrast to the minor groove binding seen for Isw1 and predicted for Chd1, the SLIDE domain of the Chd1 DNA-binding domain contacts the DNA major groove. The majority of direct contacts with the phosphate backbone occur only on one DNA strand, suggesting that Chd1 may not strongly discriminate between major and minor grooves.

Item Type:Article
Source:Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology.
Keywords:Chromatin Remodeling; Crystal Structure; DNA; DNA-binding Protein; Nucleic Acid Structure; Chd1; Iswi; Extranucleosomal DNA; Nucleosome Spacing
ID Code:83669
Deposited On:22 Feb 2012 05:14
Last Modified:22 Feb 2012 05:14

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