Fractionation and biological activities of venoms of the Indian scorpions Buthus tamulus and Heterometrus bengalensis

Ramachandran, L. K. ; Agarwal, O. P. ; Achyuthan, K. E. ; Chaudhury, L. ; Vedasiromani, J. R. ; Ganguly, D. K. (1986) Fractionation and biological activities of venoms of the Indian scorpions Buthus tamulus and Heterometrus bengalensis Indian Journal of Biochemistry & Biophysics, 23 (6). pp. 355-358. ISSN 0301-1208

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Abstract

A fractionation method, utilizing carboxymethyl-Sephadex C-25 columns buffers, for the constituents of scorpion venoms is presented. Recovery of protein from the column is high, when applied to venoms on the Indian red scorpion Buthus tamulus . The venom of Heterometrus bengalensis species could also be similarly fractionated. The former venom (i.v. LD sub(50), 3.6 mg/kg mouse) yielded two fractions both of which elicited an initial transient hypotensive effect (cholinergic) and a secondary but prolonged hypertensive effect (adrenergic) in the rat.

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