Inheritance of two somaclonal variants in mung bean (Vigna radiata (L.) Wilczek)

Abstract

Two true breeding somaclonal variants dull seed surface (Sh-tc) and green cotyledon (gc-tc) in the R₄ generation were crossed to the parental cultivar ML-5 having shiny seeds and yellow cotyledons. In the F₁ and segregating generations, dull seed surface was inherited as monogenic dominant and the green cotyledon trait was monogenic recessive. Similar segregation of the two traits independent of each other was also observed in the F₂ of the cross between the two somaclonal variants. The joint segregation was in the ratio 9:3:3:1. In addition to the parental types, as expected, a new true breeding phenotype having dull seed surface and green cotyledons was recovered. The two somaclonal variants crossed to their respective phenotypically identical radiation-induced mutants did not show any segregation, indicating that they are allelic. The results provide genetic evidence to show that monogenically inherited recessive as well as dominant mutations can be recovered from the progenies of tissue culture-raised plants of mung bean.