Chauhan, Virander S. ; Mohmmed, Asif ; Kishore, Shivendra ; Patra, Kailash P. ; Dasaradhi, Palakodeti V. N. ; Malhotra, Pawan (2005) Identification of karyopherin β as an immunogenic antigen of the malaria parasite using immune mice and human sera Parasite Immunology, 27 (5). pp. 197-203. ISSN 0141-9838
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-...
Related URL: http://dx.doi.org/10.1111/j.1365-3024.2005.00759.x
Abstract
A differential immunoscreening of the λgt11 Plasmodium falciparum genomic expression library was carried out using anti-P. yoelii sera (convalescent-phase mouse sera) and immune sera collected from healthy adults, to identify novel cross-reactive and possibly protective antigens of the parasite. One clone, with an insert size of 1132 bp that reacted strongly with both the sera was selected. The insert was found to be a part of the P. falciparum karyopherin β (PfKβ) homologue. RT-PCR and Northern blot analysis confirmed the expression of PfKβ in the blood stages of the parasite. The ~110 kDa protein was localized in the cytoplasm at the ring and trophozoite, and in the parasitophorous vacuole at the schizont stage. Two large fragments of PfKβ representing the N- and C-terminal halves were expressed in E. coli. The recombinant proteins were highly immunogenic in mice, and also found to be the target for immune response in natural infections of Plasmodium spp. Anti-sera against the protein showed a low level of anti-parasitic activity. Immunization with recombinant PfKβ fragments was only partially protective against a heterologous challenge infection in mice. Our results show that the parasite releases a highly immunogenic, cytoplasmic protein into the host which may not contribute to the development of protective immunity.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons, Inc. |
Keywords: | Immune sera; Immunogenicity; Karyopherin β; Plasmodium falciparum |
ID Code: | 8225 |
Deposited On: | 26 Oct 2010 12:09 |
Last Modified: | 16 May 2016 18:16 |
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