Hoque, Kazi Mirajul ; Pal, Amit ; Balakrish Nair, G. ; Chattopadhyay, Sukumar ; Chakrabarti, Manoj K. (2001) Evidence of calcium influx across the plasma membrane depends upon the initial rise of cytosolic calcium with activation of IP3 in rat enterocytes by heat-stable enterotoxin of Vibrio cholerae non-O1 FEMS Microbiology Letters, 196 (1). pp. 45-50. ISSN 0378-1097
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...
Related URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10538.x
Abstract
In response to heat-stable enterotoxin of Vibrio cholerae non-O1, the initial rise of cytosolic Ca2+ occurred with activation of IP3. Chelation of extracellular Ca2+ with EGTA and suspension of cells in Ca2+ free buffer both demonstrated the involvement of internal stores in the rise of [Ca2+]i. Cells pretreated with dantrolene resulted in decrease of [Ca2+]i response which suggested that the rise of intracellular level of Ca2+ was mostly due to the mobilization from IP3 sensitive stores. When the cytosolic Ca2+ was chelated by loading the cells with BAPTA, NAG-ST could not induce Ca2+ entry to the cell as assessed by Mn2+ quenching of fura-2 fluorescence which suggested that calcium influx across the plasma membrane depends upon initial rise of this bivalent cation that maintained the sustained phase of [Ca2+]i response. Addition of toxin to the fura-2-loaded cells, preincubated with lanthanum chloride, resulted in reduction of [Ca2+]i level with a short duration of irregular sustained phase further suggesting that the influx of Ca2+ across the plasma membrane might be through the calcium channel.
Item Type: | Article |
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Source: | Copyright of this article belongs to John Wiley and Sons. |
Keywords: | Heat-stable Enterotoxin; Calcium; Inositol Triphosphate; Rat; Enterocyte; Vibrio cholerae Non-O1 |
ID Code: | 82005 |
Deposited On: | 08 Feb 2012 13:20 |
Last Modified: | 17 Jul 2012 12:59 |
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