Time resolved fluorescence spectroscopy: tres and tranes

Abstract

Time resolved area normalized emission spectroscopy (TRANES) is one-step forward from TRES which is the current textbook standard for presenting fluorescence emission spectra during the lifetime of the excited state. ":''lANES helps to identify the number of emissive species in a sample. TRES and TRANES together make . The interpretation of the fluorescence spectra unambiguous. Most importantly. some pitfalls in the use of TRES to interpret the excited state phenomena in complex chemical and biologi~al systems are overcome if the analysis is extended to TRANES. This paper reviews TRES and TRANES with many examples to demonstrate the usefulness of TRANES for the interpretation of fluorescence data in complex systems.