Evaluation of the multitest medium for rapid presumptive identification of Vibrio cholerae from environmental sources

Nair, G. B. ; Misra, S. ; Bhadra, R. K. ; Pal, S. C. (1987) Evaluation of the multitest medium for rapid presumptive identification of Vibrio cholerae from environmental sources Applied and Environmental Microbiology, 53 (5). pp. 1203-1205. ISSN 1098-5336

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Official URL: http://aem.asm.org/content/53/5/1203.abstract

Abstract

The multitest V. cholerae medium (VC medium) for rapid presumptive identification of Vibrio cholerae was evaluated. On the basis of reactions in the VC medium, 379 strains recovered during a yearlong ecological study in Calcutta were presumptively identified as V. cholerae. Further phenotypic characterization of these strains revealed that the reactions of 371 (97.9%) isolates were consistent with that of V. cholerae. False-positive reactions were exhibited by eight (2.1%) strains, three of which were identified as Vibrio fluvialis biotype 1. By slightly varying the basic formulation of the VC medium, we could eliminate some false-positive reactions. On the basis of the present evaluation, we recommend the routine use of the VC medium.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiolog.
ID Code:80854
Deposited On:02 Feb 2012 09:25
Last Modified:02 Feb 2012 09:25

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