Yoshino, Ken-ichi ; Miyachia, Miki ; Takao, Toshifumi ; Bag, Prasanta K. ; Xiaozhe, Huang ; Balakrish Nair, G. ; Takeda, Tae ; Shimonishi, Yasutsugu (1993) Purification and sequence determination of heat-stable enterotoxin elaborated by a cholera toxin-producing strain of Vibrio cholerae O1 FEBS Letters, 326 (1-3). pp. 83-86. ISSN 0014-5793
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0014-5793(93)81766-S
Abstract
Four molecular species of heat-stable enterotoxins elaborated by a cholera toxin-producing strain of Vibrio cholerae O1 were isolated from its culture supernatant. The amino acid sequence of one of the enterotoxins was determined to be Phe-Ile-Lys-Gln-Val-Asp-Glu-Asn-Gly-Asn-Leu-Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn with three intramolecular disulfide linkages. The other enterotoxins had shorter amino acid sequences in the N-terminal regions, but possessed the same sequence in their C-terminal regions including the three disulfide linkages. The enterotoxins with the shorter N-terminal sequences showed more potent toxicities, and the minimum effective dose of the longest one with 28 amino acid residues was 10-folds of that of the shortest one.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Heat-stable Enterotoxin; Primary Structure; Cholera; Vibrio Cholerae O1 |
| ID Code: | 80811 |
| Deposited On: | 02 Feb 2012 03:59 |
| Last Modified: | 02 Feb 2012 03:59 |
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