Electrophoretic mobility and immune response of outer membrane proteins of Vibrio cholerae O139

Pal, Sangita ; Sasmal, D. ; Guhathakurta, B. ; Nair, G. B. ; Bhattacharya, S. K. ; Mallick, R. ; Datta, A. (1996) Electrophoretic mobility and immune response of outer membrane proteins of Vibrio cholerae O139 FEMS Immunology and Medical Microbiology, 15 (2-3). pp. 143-148. ISSN 1574-695X

Full text not available from this repository.

Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...

Related URL: http://dx.doi.org/10.1111/j.1574-695X.1996.tb00065.x

Abstract

The outer membrane (OM) protein components of a Vibrio cholerae O1 and four V. cholerae O139 strains, collected from cholera patients, were analysed by SDS-PAGE. A protein of 69 kDa molecular mass was observed only when the OMPs were prepared from strains grown in synthetic broth. As a result of passage in the rabbit ileal loop (RIL), virulence was enhanced, and a protein component around 18 kDa of the V. cholerae O139 OM became the major protein component. On immunoblot analysis with rabbit antiserum against V. cholerae O139 OM, it was shown that, apart from the major protein component of V. cholerae O1 OM of around 45 kDa and that of V. cholerae O139 OM of around 38 kDa, all other minor protein components were cross-reactive between the two serogroups. In immunoblot assays with convalescent sera obtained from V. cholerae O139-infected patients, it was observed that in addition to the lipopolysaccharide (LPS)-induced antibody, only the 38 kDa major protein component elicited considerable levels of antibody in the pateint. Minor OM components of 18 kDa were detected in the immunoblot analysis by LPS-directed antibody, however, as the OM proteins are known to be associated with LPS.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Vibrio Cholerae O139; Outer Membrane Protein; vibrio Cholerae O1; Lipopolysaccharide
ID Code:80756
Deposited On:02 Feb 2012 04:08
Last Modified:02 Feb 2012 04:08

Repository Staff Only: item control page