Srilakshmi, Gollapudi Venkata ; Sen, Joyeeta ; Chaudhuri, Arabinda ; Ramadas, Yerramsetti ; Rao, Nalam Madhusudhana (2002) Anchor-dependent lipofection with non-glycerol based cytofectins containing single 2-hydroxyethyl head groups Biochimica et Biophysica Acta: Biomembranes, 1559 (2). pp. 87-95. ISSN 0005-2736
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00052...
Related URL: http://dx.doi.org/10.1016/S0005-2736(01)00442-4
Abstract
Detailed structure-activity investigations aimed at probing the anchor chain length dependency for glycerol-based lipofectins have been reported previously. Herein, we report on the first detailed investigation on the anchor-dependent transfection biology of non-glycerol based simple monocationic cytofectins containing single 2-hydroxyethyl head group functionality using 11 new structural analogs of our previously published first generation of non-glycerol based transfection lipids (lipids 1-11). The C-14 and C-16 analogs of DOMHAC (lipids 4 and 5, respectively) were found to be remarkably efficient in transfecting COS-1 cells. In addition, the present anchor-dependency investigation also revealed that the C-14 analog of DOHEMAB (lipid 10) is significantly efficient in transfecting both COS-1 and NIH3T3 cells. Our results also indicate that too strong lipid-DNA interactions might result in weaker transfection for non-glycerol based cationic lipids. In summary, the anchor-dependence investigations presented here convincingly demonstrate that non-glycerol based cationic lipids containing a single hydroxyethyl head group and hydrophobic C-14 or C-16 anchors are promising non-toxic cationic transfection lipids for future use in liposomal gene delivery.
Item Type: | Article |
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Source: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Cytofectin; Cationic Lipid; Lipofection; Lipid-DNA Interaction; Transfection |
ID Code: | 8070 |
Deposited On: | 25 Oct 2010 10:15 |
Last Modified: | 31 Jan 2011 09:08 |
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