Interfacial indazolization: novel chemical evidence for remarkably high exo-surface pH of cationic liposomes used in gene transfection

Banerjee, Rajkumar ; Das, Prasanta Kumar ; Chaudhuri, Arabinda (1998) Interfacial indazolization: novel chemical evidence for remarkably high exo-surface pH of cationic liposomes used in gene transfection Biochimica et Biophysica Acta: Biomembranes, 1373 (2). pp. 299-308. ISSN 0005-2736

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00052...

Related URL: http://dx.doi.org/10.1016/S0005-2736(98)00113-8

Abstract

Cationic liposomes are used as the carriers of polyanionic genes for combating against hereditary diseases in gene therapy. Studies directed to careful biophysical characterizations of the cationic liposomes commonly used in gene delivery have just begun. Herein, we report on a novel liposomal exo-surface bound indazolization reaction of an amphiphilic arenediazonium salt as evidence for the existence of remarkably alkaline exo-surface of cationic liposomes commonly used in gene transfection. Our results demonstrate that formation of 5-hexadecyl-7-methylindazole in thermla indazolization of 2,6-dimethyl-4-hexadecylbenzenediazonium tetrafluoroborate bound to liposome surface is a strong indication for the existence of significantly high exo-surface pH for cationic liposomes commonly used in gene delivery. The present method can be used in determining the relative exo-surface basicities of various cationic liposomes used in gene transfection and subsequently to find any possible correlation between the transfection efficiencies of these liposomes and their exo-surface basicities.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Gene Transfection; Cationic Liposome; Surface pH; Interfacial Indazolization
ID Code:8067
Deposited On:25 Oct 2010 10:14
Last Modified:31 Jan 2011 09:24

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