Transcriptional bursts: a unified model of machines and mechanisms

Abstract

Transcription is the process whereby RNA molecules are polymerized by molecular machines, called RNA polymerase (RNAP), using the corresponding DNA as the template. Recent in vivo experiments with single cells have established that transcription takes place in "bursts" or "pulses". In this letter we present a model that captures not only the mechano-chemistry of individual RNAPs and their steric interactions but also the switching of the gene between the ON and OFF states. This model accounts for the statistical properties of the transcriptional bursts. It also shows how the quantitative features of the distributions of these bursts can be tuned by controlling the appropriate steps of operation of the RNAP machines.