Single additional methylene group in the head-group region imparts high gene transfer efficacy to a transfection-incompetent cationic lipid

Singh, Rajkumar Sunil ; Chaudhuri, Arabinda (2004) Single additional methylene group in the head-group region imparts high gene transfer efficacy to a transfection-incompetent cationic lipid FEBS Letters, 556 (1-3). pp. 86-90. ISSN 0014-5793

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Official URL: http://dx.doi.org/10.1016/S0014-5793(03)01381-4

Related URL: http://dx.doi.org/10.1016/S0014-5793(03)01381-4

Abstract

In combination with equimolar 1,2-dioleoyl-L-α-glycero-3-phosphatidyl ethanolamine, a novel cholesterol-based cationic lipid with β-alanine head-group (2) has been demonstrated to be strikingly more efficacious (10-24-fold) in transfecting CHO, COS-1 and HepG2 cells than its glycine analog (1) containing just one less methylene unit in its head-group region. Syntheses, characterizations and in vitro transfection biology of lipids 1 and 2 are described. Present findings demonstrate that even truly minor structural alterations, such as inclusion of just one additional methylene functionality in the polar head-group region, can convert an essentially transfection-incompetent cholesterol-based cationic amphiphile to a remarkably efficient cationic transfection lipid.

Item Type:Article
Source:Copyright of this article belongs to Federation of European Biochemical Societies.
Keywords:DNA Transfection; Cytofectin; Lipoplex; Cholesterol-based Cationic Amphiphile; Lipofection; Transgene Expression
ID Code:8064
Deposited On:25 Oct 2010 10:13
Last Modified:16 May 2016 18:08

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