Rapid detection of early typhoid fever in endemic community children by the TUBEX® O9-antibody test

Rahmana, Mahbubur ; Siddique, Abul Kasem ; Tam, Frankie Chi-Hang ; Sharmin, Sabrina ; Rashid, Harunur ; Iqbal, Anwarul ; Ahmed, Sirajuddin ; Nair, Gopinath Balkrish ; Chaignat, Claire-Lise ; Lim, Pak-Leong (2007) Rapid detection of early typhoid fever in endemic community children by the TUBEX® O9-antibody test Diagnostic Microbiology and Infectious Disease, 58 (3). pp. 275-281. ISSN 0732-8893

Full text not available from this repository.

Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/j.diagmicrobio.2007.01.010

Abstract

Typhoid remains a global public health problem, and quick accurate immunodiagnosis is needed. Here, we examined the performance of the 5-min TUBEX® O9-antibody detection kit in 243 outpatients (mostly children and infants) in their first week of fever and 57 healthy subjects in the Bangladesh community. Based on culture results, TUBEX® was 91.2% (31/34) sensitive and 82.3% (172/209) specific in febrile subjects. However, specificity was better in nonfebrile healthy subjects (89.5%, 51/57) or in febrile individuals who serologically had dengue fever (90.5%, 57/63), suggesting that some culture-negative febrile individuals could be truly typhoidal. These individuals were also positive in an anti-crude O9 enzyme-linked immunosorbent assay (ELISA) and the Widal test. Regression analysis of the TUBEX® and ELISA results showed good concordance between them, better with the combined IgM-IgG ELISA than with IgM alone, suggesting that TUBEX® detects IgM antibodies not necessarily by themselves, as previously reported, but with the help of IgG antibodies.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Immunodiagnosis; Infectious Disease; Salmonella
ID Code:80621
Deposited On:01 Feb 2012 12:52
Last Modified:01 Feb 2012 12:52

Repository Staff Only: item control page